All readings were taken in clear, polystyrene 96-shallow-well, flat-bottom plates. 3% in white 96- and 384. When samples are added to the wells, the Protein G molecules are resuspended and binding occurs. 4D-Nucleofector 96-well Shuttle: RATE CONTROLLED. x. Standard 6-, 12-, 24-, 48-, 96- and 384-well microplates. Top or bottom detection is available with a simple click of a button. Industry standard, 96-well plates. Microplate Spectrophotometers. Calcium flux was measured with a FlexStation 3 Microplate Reader using the Flex (fast kinetic) read type. Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. M2. On the FlexStation 3 reader, the entire series of reactions is monitored in real time with simultaneous on-board reagent pipetting and luminescence detection (Figure 1). The SpectraMax ABS Plus reader was heated to 37ºC prior to setting up the assay. The Best Microplate Readers According to our Product Reviewers (1) Write a Review. The luminescence was measured in a SpectraMax. The EMax Endpoint ELISA Microplate Reader from Molecular Devices (one of the pioneers of microplate detection) provides a simple and durable dedicated endpoint detection method. Resipher 96x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 96x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 96 wells Utilizes Lucid Lab Cloud software for connection to any…. For the 96-well plate, this equates to 1 fg per well to 1 ng per well; for the 384-well plate it is 0. and California)The upgradeable SpectraMax M5 Multi-Mode Microplate Reader comes with the standard five modes of detection with the upgrade option to add HTRF at a later time. Cell line development teams will realize savings on time and resources, as well as faster time-to-market by utilizing a single technology for measuring a critical quality attribute duringOpening the Drawer Manually on page The SpectraMax ABS can accommodate standard 96-well plates. The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. We would like to show you a description here but the site won’t allow us. Whether running fluorescence imaging or brightfield assays, the automated imager features a comprehensive portfolio of preconfigured protocols for cell-based assays to shorten the learning curve, so. They are also sealable using different types of adhesive mats foils or films. 2 OD at 200 nm; this decreased to 0. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. . Tecan’s quality control and validation packages help our customers to fulfill all regulatory requirements for their Tecan microplate readers in an efficient, cost-effective way. Exposure of ApNA to FAAH activity results in the release of the yellow colorimetric dye p-nitroaniline (ε = 13,500 at 382 nm). Includes PC and software. The CellTiter-Glo assay was used to measure ATP. Therefore you need to ask a physicist. • Specially formulated optically clear polystyrene and treated to provide protein coating capacity. x. The SpectraMax® M2 and M2e Systems from Molecular Devices are multi- detection microplate readers with dual- monochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). The reader includes multiple licenses to the SoftMax® Pro software for endpoint and kinetic analysis of microplate. 500 OD ± 3. This offers the potential for fast and convenient measurements of FAAH activity using a 96-well plate spectrophotometer. We would like to show you a description here but the site won’t allow us. 020 fmol 0. $14,995. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Quartz Plate found in: Thick Quartz Ground and Polished Plates, Solid Quartz 96 Well Clear Plate with Clear Base, Quartz Parallel Plate Probes for DMA 7. M2. a white 96-well half-area microplate (Corning cat. Wells of 96-well plates (NEST) were pre-coated with 100 μg/ml of collagen solution for 1 hour. 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). VIS absorbance detection. Create custom fluorescence assays without having to purchase expensive filter sets. 1. ₹ 1,80,000 Get Latest Price. Important things to consider when purchasing a multimode microplate reader. Top reading gives greater sensitivity for 96 well plates, unless you have cells adhering to the bottom of the well. Step 2: Add sample to well – antigen within the sample binds to the capture antibody. With more than 40 years of plate reader expertise and life science research, we’ve amassed an extensive. style. 0 OD. 96-, 384-, and 1536-well microplates. Part of a plate can be selected so that only a selected area of the plate. Microplate readers, SpectraMax®, with SpectraDrop. The procedures in this protocol support the 96-well reaction plate. x. Next-generation luminescence plate reader. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and. Description Bottom Volume Qty/Pk Qty/Cs 3635 96 Well UV-Plate Flat 370 µL 2550per well of this semi-solid mixture was pipetted into 6-well plates. Cells were transfected as previously described and plated at 30,000 cells per well in a 96-well plate and grown overnight. 5% at 1. 96- and 384-well. 1. #i3x) SpectraMax L Microplate Reader (Molecular Devices cat. 20 µL (96-well plates) or 10 µL (384. Ensure that cells reach around 80–90% of confluence on the day of the. More Product Information. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. #R8340) SpectraMax i3x Multi-Mode Microplate Reader (Molecular Devices cat. White 96-well plate Plate formats. The EMax® Endpoint ELISA Microplate Reader from Molecular Devices, one of the pioneers in microplate detection, offers a ruggedly simple approach to dedicated endpoint assays. Look for a washer which can handle a variety of plate types, including deep well plates and 96-, 384- and 1536-well plates. Spectramax ® Plus microtiter plate reader (Molecular Devices, Sunnyvale, CA) or similar UV-Vis plate reader; SoftMax ® Pro software (Molecular Devices) (optional) UV compatible quartz plate (for example: # R8024; Molecular Devices) or 96-well Costar ® with UV transparent bottom (for example: #3635 ; Corning Inc. Figure 1-2 Match chamfered corners of slide and adapter 2. SpectraMax iD5 Multi-Mode Microplate Reader (Molecular Devices cat. com Molecular Devices (Germany) GmbH ismarcring ierach an der iss erman ©2019 Molecular Devices, LLC. is required for optimum performa nce with standard 96-well and 384-well format microplates for all top-read applications. Published Dec 16, 2020 | Updated Jun 22, 2023. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader. An industry-renowned instrument for monitoring GPCRs and ion channels. Usage/Application: Lab. No accessories are needed and SoftMax® Pro Software. Optional 12-way manifold) 5032336. 100 uL of LAL reagent was then added to the wells containing the endotoxin standards, and the kinetic read was started. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. A = Log 10 (I 0 /I). Powerful, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers. PURPOSE. Time-resolved phosphorescence. The system features 96, 384,. Max, FlexStation, Gemini, and earlier instruments as well as import and analysis of data files from FLIPR and Analyst instruments. x. x. Molecular Devices' microplate readers. . 96-well Molecular Devices P/N 42-000-0117 HE™ High Efficiency microplates, 384-well Molecular Devices P/N 0200-5201. The SpectraMax ABS Plus, can accommodate standard 96-well plates and 384-well plates. A barcode reader accessory can be purchased with theWe would like to show you a description here but the site won’t allow us. Each well of a 96- well microtiter plate is coated with a fluorescently labeled IgG-binding peptide, Protein G. The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. Working in. Reads various kinds of 96-wells plates, and is equipped with shaking function. Our microplate. Absorbance Linearity: 2. Microplate readers from Tecan; cutting edge bench-top microplate readers lead the industry in both versatility and performance. Microplate readers compatible with 96-well plates Single- to multi-mode readers combine an absorbance plate reader , a luminescence plate reader , and a fluorescence plate. the use of a reader calibrated with primary NIST and NMI standards. Get Quote . The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life. #6590-0137) Optimal instrument settings for ValitaTiter (P/N VAL003 or VAL013, left table) and ValitaTiter Plus (P/N VAL004 or VAL014, right table) assays on the SpectraMax iD5 reader are shown below. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. In labs where throughput is a top priority, a device that can screen 96- to 1536-well microplates in under one minute is worthwhile. Partner with our team of scientists and engineers who can customize your instruments, as well as automate entire workflows to meet the specific needs of your assay, method, or protocol. 1% in white 96- and < 0. Meanwhile, Cowan discusses the importance of considering plate well densities and the angle from which microplates are read inside the reader: “You should definitely check the range of plate well densities supported by your candidate readers. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. Contents of wells were pipetted up and down several times to mix. of Dispensers:Molecular Devices deliver high throughput while showing good correlation with manual. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for. Molecular Devices' microplate readers. To assess the sensitivity of each microplate reader, a plate was set up with 32 wells containing PBS only. 96-well-plate-scan-time-2-colors-1-site-well. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories involved in many applications. An entire 96-well plate can be processed in less than six seconds. The FilterMax™ F3 features absorbance (visible), fluorescence intensity - FI (top reading), and glow luminescence for 96- to 384-well plates. The instrument reads any standard cuvette, microcuvette, test tube and 96 well or UV transparent microplate over full 190-1000 nm wavelength range. Click Create. T = I/I 0 and %T = 100 (T). An additional reference channel for optimizing the light intensity; Auto switch-off of the light source when not in use to extend lifetime; An automated self-check of major components at instrument start-upThe EMax® Plus Microplate Reader delivers a robust solution in an entry level platform. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the. Locate the User Guide on your SoftMax Pro computer for version 6 and version 7. 45 µm PVDF. Washer maintenance kit: 2 X T 2. x. Polypropylene Deep well Plates are used as small test tubes because of easier sealing, flat or conical shape, square and round well formats with lids and cap. Fluorescence Microplate Reader Comparison Chart. 20 attomol ATP per well. Cross-talk <0. Top and bottom reads are. com. The device was able to achieve 3σ limits of detection of ca. Other technologies such as imaging. The optical system of the SpectraMax 190 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the UV-visible wavelength range (190 nm - 850 nm). PURPOSE. Streamline multiple steps (imaging, sample tracking, data analysis and report generation) in the cell line development workflow for IND filing. Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Temperature control: 4°C above ambient to 45°C. A polyclonal antibody specific to mouse/rat IL-22 was pre-coated onto a 96-well microplate. Molecular Devices Tunable Microplate Reader VersaMax $ 1,495. Corning. Use form “26003-02: Molecular Devices Plate Reader Plate Calibration Form” to record material and equipment information. MCF7 cells treated with. Temperature control (RT+3°C – 42°C) Liquid dispensers with reagent heater and stirrer. Dual injectors for kinetic assays. The Absorbance 96 Plate Reader is designed to run everyday assays with ease and speed. Products : SpectraTest Validation Plates ABS2, SpectraTest Validation Plates ABS1, SpectraTest Validation Plates FL1, SpectraTest Validation Plates LM1. Selects either 96-well or 384-well plate for a stand-alone read. Highly selective for dsDNA over ssDNA or RNA. Mix well by trituration or plate shaker and incubate for 2 to 5 minutes at room temperature, protected from light. Quickly image a variety of plate formats and cell types - Image a 96-well plate in under two minutes. These lab workhorses are used in drug discovery. 000 to 2. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. The plate reader can measure multiple readouts, including UV and. Luminescence applications and assays. . SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. Compatible with Molecular Devices SPECTRAmax 190 and 250 plate readers. Molecular Devices has provided scientists with . Order Now. 96-, 384-, and 1536-well microplates. . If flexibility becomes important in a growing lab supporting a variety of projects, the decision might come down to whether. Published Dec 16, 2020 | Updated Dec 06, 2021. Features of Streptavidin Coated Plates include: • Approximate binding capacity is ∼5 pmol biotin/well. $3,450. Ultra-cooled Photomultiplier Tube and : Silicon photodiode. Page 1 of 8 . The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular biology and immunoassays. 1c shows a cover designed to perfuse 8 wells in series on a standard 96-well microplate, as well as a cover for the entire standard 96-well microplate. * Note: Programming the washer with a final aspirate height of 4. Answer. UV Plate 96 Well, Flat Bottom, Cs 50. The BCA assay plate was read at 562 nm, the original wavelength setting in the protocol, while for the BCA Rapid. • Photometric range: 0. S. 00. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. In addition, the iD5 reader measures TRF and FP and. Plate formats 6, 12, 24, 48, 96, 384 wells Light source Xenon flash lamp (1 joule/flash) Detector Photomultiplier (R-3896). Visit the supplier page or submit a quote to learn more. White plates reflect the light, enabling even low levels of Luminescence to be detected. Aspirating and dispensing of wash buffer can occur simultaneously in all wells via AquaMax® 4000 Microplate Washer with a 96-. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. Corning. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M4 Microplate Reader transforms each well in a microplate to a fixed. Mix samples in microplate wells by user-programmable Automix feature. d. Dimensions: 18. For the name of the nearest authorized Molecular Devices Corporation service facility please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. Well scans from the Gemini EM Reader illustrating serial dilution of CHO cells using the Live Cell assay from Molecular Probes Live/Dead (Calcein AM/Ethidium Homodimer-1) assay kit. The SpectraMax® M2 and M2e Systems from Molecular Devices are multi- detection microplate readers with dual- monochromators, dual-mode cuvette ports, and top- and. SpectraMax 250 User. The approximate dead volumes are: 96-well plates: V-bottom: 20-30 µl U-bottom: 30-40 µl flat-bottom: 50-60 µl 384-well plates: approximately 20 µl. At 5 seconds to read a 96-well endpoint assay or a 5 second minimum interval between kinetic reads, the VMax Microplate Reader has both speed and performance. SoftMax Pro Software minimizes setup time for detection and automates standard curve fitting and sample quantitation. 384-WELL AND 96-WELL 10MM-HEIGHT STERILE MICROPLATES. The AquaMax® Microplate Washer is a fully self-contained system, configurable for both 96- and 384-well microplates. Microplate optimization and read height optimization were performed for best results. Kinetic readings were started immediately with a Molecular Devices FlexStation Plate Reader. Important things to consider when purchasing a multimode microplate reader. Can read 96 well plates (optional 384 well). HepG2 cells were plated into a 96-well clear-bottom, white-walled plate at 10,000 cells/well and incubated at 37°C overnight. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Features. 2. 9. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. The VersaMax Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible. Built around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. 046 to 0. Washers configured for both 96- and 384-well plates. It offers extremely low absorbance background, excellent well to well uniformity, and zero percent cross talk. May require LS-50B…. Running both standard spectrophotometer and microplate. SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. #SpectraMax L Config) Methods. minutes and begin reading microplates with seven mouse clicks. 005 OD Normal and Rapid modes, 96- and 384-well plates;. Erik Koppes, Richard Haas, Jerry Vockley, Lina Ghaloul-Gonzalez approximately 5,000 cells were seeded in 4 replicates in 96-well plates. It also integrates with the. A Costar® 24-well tissue culture plate with col1 = blanks and doubling cell concentration left to right starting at Col. 0% ± 0. Learn how you can utilize microplate readers to drive landmark discoveries. With theMolecular DevicesWe designed and fabricated different sized microfluidic covers, matched to the desired experimental conditions: Fig. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. Service Contracts, Preventative Maintenance and. e 3370 Costar 96 Well. 96-, 384-, and 1536-well microplates. LogPhase 600 is controlled with a software App that provides a simple, yet powerful, user experience. x. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the. #C1500) Control standard endotoxin (ACC cat. This SpectraMax Plus comes from a long history of reliable tools built by Molecular Devices. The. 340 PC384. FLIPR System with the 540-590 nm BP Emission FLIPR Filter installed (Molecular Devices Corporation) Membrane Potential Assay Kit (Molecular Devices Corporation, R-8034) Clear, flat-bottom, black- or clear-wall 384- or 96-well plates (see consumables list in Appendix A) Clear, polypropylene compound plates (see consumables list in Appendix A) Flexibility to read a variety of plate formats. Description. A. Well scanning With well scanning, multiple points within each well can be read, providing a high. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Elevated well walls for easier sealing Round bottom formats Square and round well formats Conform to SBS standards Lids and cap mats available Packed in sleeves of 6, 24. A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and screening of drug compounds and targets, among others. FilterMax F3 and F5 Multi-Mode Microplate Readers are filter-based microplate readers offering fluorescence polarization, time-resolved fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. Shaking: Linear, orbital, and double orbital. 96-well microplate compatible Well read area range: D4. XTR Rack Pro 96 (Vial Rack Bottom Label 2D Reader) BIO-LAYER INTERFEROMETRY MICROPLATE READING: Forte Bio: Octet HTX: Octet QK384:. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. 984. #N207) LAL Reagent Water (Lonza cat. Flexible liquid transfer Using an 8- or 16-channel pipettor, the FlexStation 3 Reader offers added assay flexibility over dispenser-based systems by transferring reagents from 96 or 384 distinct wells in a source plate to a read plate, simultaneously. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Temperature variation ± 0. The screen on the left shows the wells selected for Method MI AF488-10 and Method MI AF488-5 for image acquisition from rows A-D of a 96-well plate at. Pick the best speed, sensitivity, and price for your needs. Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. 00 . The plate was preincubated in the reader at 37°C for a minimum of 10 minutes. g. Plate chamber design of the SpectraMax category 1 microplate readers. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. We would like to show you a description here but the site won’t allow us. Resipher 96x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 96x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 96 wells Utilizes Lucid Lab Cloud software for connection to any…. To help you get the best results from your Molecular Devices® plate readers it is essential that you choose the right plate. Reader Documentation Version No. Microplates, also known as microtiter plates, are rectangular multi-well plates used in a variety of assays from ELISA to PCR. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. # SMP54-GXP- 10UL) Pyrochrome Assay chromogenic test reagent (ACC cat. 0 nm increments Dynamic range > 7 decades Cross-talk < 0. 3. Bowman Dispensers. QuickSync. 96/384-well plate formats. Materials. 200, the Bio-Tek µQuant™, the Bio-Rad UltraMark™, the Molecular Devices SPECTRAmax® 190 and 250 plate readers. The VICTOR Nivo. sdale innersh Triangle Woingham ershire RG41 T United Kingdom 11 umolde. An enzyme-linked polyclonal antibody specific for mouse/rat IL-22 was added to the wells and. 130009WMicroplate Readers. 2. Cell viability and cytoxicity assays. Figure 1. Molecular Devices SpectraMax Plus Microplate Reader Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader Sale Price: $6,495. Z. * Note: Programming the washer with a final aspirate height of 4. An industry-renowned instrument for monitoring GPCRs and ion channels. FlexIII. #ID5-STD) Pierce BCA Protein Assay Kit (ThermoFisher Scientific cat. Compare. A custom 96-well plastic (polyethylene terephthalate/PET) plate is attached via epoxy to the mounted CMOS ICs, designed with a standard form factor to be compatible with high-throughput. UV absorbance detection. We have the following plate-reader instrument available for on-site screens and other projects Molecular Devices Spectramax Paradigm for luminescence, fluorescence, and. Kinetic assays were read every 20-28 seconds for one hour,The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Applications include ELISA, protein quantification, enzyme kinetics and cell-based assays. The opaque walls prevent stray light from being detected from neighbouring. 4 (Molecular Devices cat. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. # SMP54-GXP- 10UL) Pyrochrome Assay chromogenic test reagent (ACC cat. 2% in. OD measurements of bacterial and colloidal bead cultures were performed in a Spectrostar Omega microplate reader (BMG, Germany) with a Costar Flat Bottom 96-well plate with lid and 200 µl per. Narrow bandwidths deliver increased measurement accuracy and linearity over the widest range of assays, including. 24-, 96-, 384-well plates and microwell strips; Manifold Channels: 96-well washing: 8-well (1x8) manifold, 2x8-well manifold, 12-well (1x12) manifold, 8-well short tube (1x8) manifold. The ELx808 TM Reader seamlessly interfaces with Lonza's WinKQCL TM Endotoxin Detection and Analysis Software. White 96-well platePlate formats. Access this by going to. x. Contains one microplate adapter, five 24-well low volume slides, five 64-well low volume bottom slides, and five of each cover slide (2 µL and 4 µL) 0200-6267. Plate. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384. FlexStation® 3 Benchtop Multi-Mode Microplate Reader combines Molecular Devices’ SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. This is equivalent to having 511 built-in filters, The instruments offer 96- and 384-well microplate-to-microplate fluid transfer (8 or 16 wells at a time), and can read microplates having 6, 12, 24, 48, 96, and 384-well formats. Luminometers and fluorimeters allow for readout of luminescence (luciferase readouts) or fluorescence from an entire well of a micro-well plate such as a 96- or 384-well plate. 5 pM Luminescence performance Wavelength range 300–850 nm Wavelength selection Choice of simultaneous detection of all wavelengths or selection in 1. Read the microplate • Make sure the purple plate adapter (if required for your plate reader) is in the microplate reader drawer. Calcium flux was measured with a FlexStation 3 Microplate Reader using the Flex (fast kinetic) read type. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. Accurate, efficient nucleic acid quantitation is critical for molecular biology labs that work with precious, rare, or difficult-to-process samples and applications requiring precise measurement. SpectraTest FL1 Fluorescence Validation Plate User Guide Molecular DevicesData are shown as the mean ± s. On the FlexStation 3 reader, the entire series of reactions is monitored in real time with simultaneous on-board reagent pipetting and luminescence detection (Figure 1). Multimode microplate reader equipped with latest detections like fluorescence,. The SpectraMax Paradigm Platform was able to quantify the protein down to the low picomolar level using an AlphaLISA method (Figure 1). validation, maintenance, and repair documentation. UV absorbance detection. Users can also perform measurements such as endpoint, multiple wavelength, kinetic, linear and area scans. 90349). CO 2 & O 2 control. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. to each well. Sensitive luminometer with programmable injector options for 96- and 384-well microplates. plate loading adapter and a choice of magazines for 20, 40 or 50 SBS-standard 96- and 384-well microplates. Selects either 96-well or 384-well plate for a stand-alone read. MultiWash+ for 96-well plates: 4 liquid lines, 8-way manifold and tubing, 4 wash/rinse bottles (2L), 1 waste bottle (2. Features of Clear 96-Well Microplates: • Clear plates for colorimetric ELISA and other assays. 0. M5. Figure 3. 2. 0% ± 0. Lucid Scientific. The onboard luminescence detection mode may be used, or, for higher sensitivity, the reader can be equipped with a NanoBRET detection cartridge that offers a 4-fold improvement in limit of quantitation. 5 fg per well to 0. Making it possible to perform kinetic analysis of solid. 340 PC384. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. This reader also makes use of a unique temperature-independent methodology for path length correction. B. FAST AND ACCURATE METERTECH Microplate Reader $3,650. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0.